A Study of Radiation-Induced Instability for the Gene Locus Associated with Intellectual Disorders or Developmental Delays

Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in presence absence ionizing radiation. WTK1 cells contain a p53 mutation, whereas TK6 line has native tumor-suppressor gene. Each isolated pre- post-irradiation (2 3 Gy) analyzed by MLPA. Using probes that target specific regions on associated with distinct subset microdeletions microduplications either established or thought be responsible intellectual disability developmental delay, we have demonstrated are not impacted same way irradiation. Instead, each presents its own unique MLPA profile. The most notable differences appearance nine probe signals only seen cells. These results important how different can affected significantly ways depending wild type p53.